Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria
Identifieur interne : 00CF61 ( Main/Exploration ); précédent : 00CF60; suivant : 00CF62Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria
Auteurs : Jon B. Suzuki [États-Unis] ; Tom J. Sims [États-Unis] ; David L. Singer [États-Unis] ; Roy C. Page [États-Unis]Source :
- Journal of Periodontal Research [ 0022-3484 ] ; 1984-07.
Descripteurs français
- Wicri :
- topic : Immunologie, Constitution de société.
English descriptors
- KwdEn :
- Actinomyces viscosus, Activator, Activator concentrations, Activator dose, American academy, Amlr, Assay system, Autologous, Bacterial homogenates, Bacterial preparations, Bacterial substances, Blastogenesis, Blastogenic, Blastogenic response, Blastogenic responsiveness, Bone loss score, Cell concentration, Cell concentrations, Chronic gingivitis, Clinical immunology, Control cells, Control donors, Control subject, Control subjects, Conventional antigen, Culture conditions, Experimental medicine, Greatest extent, Human autologous, Human lymphocytes, Human periodonta, Immunology, Incorporation, Incubation time, Incubation times, Ivanyi lehner, Johns hopkins hospital, Juvenile periodontitis, Label incorporation, Lymphocyte, Lymphocyte reaction, Lymphocyte reactions, Lymphocyte transformation, Maximally, Mitogen, Oral biology, Patient cells, Patient cultures, Pediatric dentistry, Periodontal, Periodontal disease, Periodontal research, Periodontal status, Periodontitis, Peripheral blood, Peripheral blood lymphocytes, Plaque antigens, Pokeweed mitogen, Precursor, Radiographic measurements, Responsiveness, Severe periodontitis, Significant differences, Sims, Sims page, Smith lang, Spontaneous lymphocyte proliferation, Stimulation index, Suzuki, Test conditions, Unstimulated, Unstimulated cultures, Young adults.
- Teeft :
- Actinomyces viscosus, Activator, Activator concentrations, Activator dose, American academy, Amlr, Assay system, Autologous, Bacterial homogenates, Bacterial preparations, Bacterial substances, Blastogenesis, Blastogenic, Blastogenic response, Blastogenic responsiveness, Bone loss score, Cell concentration, Cell concentrations, Chronic gingivitis, Clinical immunology, Control cells, Control donors, Control subject, Control subjects, Conventional antigen, Culture conditions, Experimental medicine, Greatest extent, Human autologous, Human lymphocytes, Human periodonta, Immunology, Incorporation, Incubation time, Incubation times, Ivanyi lehner, Johns hopkins hospital, Juvenile periodontitis, Label incorporation, Lymphocyte, Lymphocyte reaction, Lymphocyte reactions, Lymphocyte transformation, Maximally, Mitogen, Oral biology, Patient cells, Patient cultures, Pediatric dentistry, Periodontal, Periodontal disease, Periodontal research, Periodontal status, Periodontitis, Peripheral blood, Peripheral blood lymphocytes, Plaque antigens, Pokeweed mitogen, Precursor, Radiographic measurements, Responsiveness, Severe periodontitis, Significant differences, Sims, Sims page, Smith lang, Spontaneous lymphocyte proliferation, Stimulation index, Suzuki, Test conditions, Unstimulated, Unstimulated cultures, Young adults.
Abstract
Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P < 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p < 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.
Url:
DOI: 10.1111/j.1600-0765.1984.tb01008.x
Affiliations:
- États-Unis
- Maryland
- College Park (Maryland), Seattle
- Université de Washington, Université du Maryland
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Le document en format XML
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Suzuki</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201</wicri:regionArea><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Sims, Tom J" sort="Sims, Tom J" uniqKey="Sims T" first="Tom J." last="Sims">Tom J. 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Singer</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201</wicri:regionArea><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Page, Roy C" sort="Page, Roy C" uniqKey="Page R" first="Roy C." last="Page">Roy C. Page</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201</wicri:regionArea><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Periodontal Research</title><title level="j" type="alt">JOURNAL OF PERIODONTAL RESEARCH</title><idno type="ISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><imprint><biblScope unit="vol">19</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="352">352</biblScope><biblScope unit="page" to="365">365</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1984-07">1984-07</date></imprint><idno type="ISSN">0022-3484</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actinomyces viscosus</term><term>Activator</term><term>Activator concentrations</term><term>Activator dose</term><term>American academy</term><term>Amlr</term><term>Assay system</term><term>Autologous</term><term>Bacterial homogenates</term><term>Bacterial preparations</term><term>Bacterial substances</term><term>Blastogenesis</term><term>Blastogenic</term><term>Blastogenic response</term><term>Blastogenic responsiveness</term><term>Bone loss score</term><term>Cell concentration</term><term>Cell concentrations</term><term>Chronic gingivitis</term><term>Clinical immunology</term><term>Control cells</term><term>Control donors</term><term>Control subject</term><term>Control subjects</term><term>Conventional antigen</term><term>Culture conditions</term><term>Experimental medicine</term><term>Greatest extent</term><term>Human autologous</term><term>Human lymphocytes</term><term>Human periodonta</term><term>Immunology</term><term>Incorporation</term><term>Incubation time</term><term>Incubation times</term><term>Ivanyi lehner</term><term>Johns hopkins hospital</term><term>Juvenile periodontitis</term><term>Label incorporation</term><term>Lymphocyte</term><term>Lymphocyte reaction</term><term>Lymphocyte reactions</term><term>Lymphocyte transformation</term><term>Maximally</term><term>Mitogen</term><term>Oral biology</term><term>Patient cells</term><term>Patient cultures</term><term>Pediatric dentistry</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal research</term><term>Periodontal status</term><term>Periodontitis</term><term>Peripheral blood</term><term>Peripheral blood lymphocytes</term><term>Plaque antigens</term><term>Pokeweed mitogen</term><term>Precursor</term><term>Radiographic measurements</term><term>Responsiveness</term><term>Severe periodontitis</term><term>Significant differences</term><term>Sims</term><term>Sims page</term><term>Smith lang</term><term>Spontaneous lymphocyte proliferation</term><term>Stimulation index</term><term>Suzuki</term><term>Test conditions</term><term>Unstimulated</term><term>Unstimulated cultures</term><term>Young adults</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actinomyces viscosus</term><term>Activator</term><term>Activator concentrations</term><term>Activator dose</term><term>American academy</term><term>Amlr</term><term>Assay system</term><term>Autologous</term><term>Bacterial homogenates</term><term>Bacterial preparations</term><term>Bacterial substances</term><term>Blastogenesis</term><term>Blastogenic</term><term>Blastogenic response</term><term>Blastogenic responsiveness</term><term>Bone loss score</term><term>Cell concentration</term><term>Cell concentrations</term><term>Chronic gingivitis</term><term>Clinical immunology</term><term>Control cells</term><term>Control donors</term><term>Control subject</term><term>Control subjects</term><term>Conventional antigen</term><term>Culture conditions</term><term>Experimental medicine</term><term>Greatest extent</term><term>Human autologous</term><term>Human lymphocytes</term><term>Human periodonta</term><term>Immunology</term><term>Incorporation</term><term>Incubation time</term><term>Incubation times</term><term>Ivanyi lehner</term><term>Johns hopkins hospital</term><term>Juvenile periodontitis</term><term>Label incorporation</term><term>Lymphocyte</term><term>Lymphocyte reaction</term><term>Lymphocyte reactions</term><term>Lymphocyte transformation</term><term>Maximally</term><term>Mitogen</term><term>Oral biology</term><term>Patient cells</term><term>Patient cultures</term><term>Pediatric dentistry</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal research</term><term>Periodontal status</term><term>Periodontitis</term><term>Peripheral blood</term><term>Peripheral blood lymphocytes</term><term>Plaque antigens</term><term>Pokeweed mitogen</term><term>Precursor</term><term>Radiographic measurements</term><term>Responsiveness</term><term>Severe periodontitis</term><term>Significant differences</term><term>Sims</term><term>Sims page</term><term>Smith lang</term><term>Spontaneous lymphocyte proliferation</term><term>Stimulation index</term><term>Suzuki</term><term>Test conditions</term><term>Unstimulated</term><term>Unstimulated cultures</term><term>Young adults</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Immunologie</term><term>Constitution de société</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P < 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p < 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region><settlement><li>College Park (Maryland)</li><li>Seattle</li></settlement><orgName><li>Université de Washington</li><li>Université du Maryland</li></orgName></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Suzuki, Jon B" sort="Suzuki, Jon B" uniqKey="Suzuki J" first="Jon B." last="Suzuki">Jon B. Suzuki</name></region><name sortKey="Page, Roy C" sort="Page, Roy C" uniqKey="Page R" first="Roy C." last="Page">Roy C. Page</name><name sortKey="Page, Roy C" sort="Page, Roy C" uniqKey="Page R" first="Roy C." last="Page">Roy C. Page</name><name sortKey="Sims, Tom J" sort="Sims, Tom J" uniqKey="Sims T" first="Tom J." last="Sims">Tom J. Sims</name><name sortKey="Sims, Tom J" sort="Sims, Tom J" uniqKey="Sims T" first="Tom J." last="Sims">Tom J. Sims</name><name sortKey="Singer, David L" sort="Singer, David L" uniqKey="Singer D" first="David L." last="Singer">David L. Singer</name><name sortKey="Singer, David L" sort="Singer, David L" uniqKey="Singer D" first="David L." last="Singer">David L. Singer</name><name sortKey="Suzuki, Jon B" sort="Suzuki, Jon B" uniqKey="Suzuki J" first="Jon B." last="Suzuki">Jon B. Suzuki</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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